Mr. Arpan Mukherjee,
Ph.D. student of Institute of Environment and Sustainable Development (IESD),
Banaras Hindu University, Varanasi, Uttar Pradesh, India. Mr. Mukherjee did his B.Sc. in Microbiology
(Hons.) from University of Burdwan and M.Sc. in Applied Microbiology and
Banaras Hindu University, India. Mr. Arpan completed 3 project as Project Assistant
in TATA Trust- USAID funded project, Junior Research Fellow in SERB-India funded project and Project Research Associate-I
in DST-India funded project. Till date he published more than 45 articles in
some reputed journals like Nature, Nat. Ecol. Evol., Nat. Com., PNAS, STOTEN,
SBB, Microbial. Res., IJEST, AMB, LDD, Agronomy etc., his total impact factor till
date is 199.192, and Google scholar citation is 721. He qualified National
Eligibility Test (NET) and GATE, he got selected in Prime Minister's Research
Fellowship (PMRF), Government of India. He was selected for “Young
Microbiologist award” by Agro Environmental Development Society (AEDS), India
in 2023; also selected for ICPP 2018 Bursary award (USA) in 2018, and get International
travel grant from BHU-IoE cell in 2022 to attend an International conference. Mr. Mukherjee is interested in developing
potential bio-inoculant for sustainable crop production. He currently working
in the area of plant microbiome, metagenomics, plant endophytes, bio-fertilizer
development. Till date Arpan trained more than 3000 farmers in different
district of Uttar Pradesh, India to promote organic farming for sustainable
agriculture production.
39
Culturable
chickpea seed bacterial endophytes: Future
of sustainable agriculture production
A. MUKHERJEE, J. P. VERMA
Lab No. 121C, Plant microbe interaction
lab, Institute of Environment and Sustainable Development, Banaras Hindu
University, Varanasi-221005, Uttar Pradesh, India
Seed endophytes is likely to have key impacts on plant growth and yield,
but the functional potentials of the seed endophytes remain very poorly
understood. It also suggested that, bio-inoculants developed from or compatible
with the seed microbiome are more likely to produce desired outcomes of
sustainable increase in agriculture productivity, but currently very few
empirical evidences are available. The aim of this research study was to
identify the culturable bacterial endophytes of chickpea (Cicer arietinum L.)
seed (P-362), and their functional attributes. We isolated total 29 culturable
bacterial strains (8 strains from dry and 21 strains from germinating chickpea (P-362)
seeds) from chickpea seeds. Molecular analysis showed that dominating seed endophytes
belong to Enterobacter sp., and Bacillus sp. Isolates produced significant amount of
Indole-3- acetic acid, solubilised phosphate and potassium, ammonia, and also
inhibited the growth of Fusarium sp.
under laboratory conditions. Reintroduction of these isolates,
resulted in significant increase in plant growth in different parameter and
bio-controlling activity against Fusarium sp. The results provide a
direct evidence for the presence of beneficial seed endophytes and suggest
these bacterial isolates could be further developed into potential bio-inoculants
for improving diseases management and sustainable increase in agriculture
productivity.